|
KMID : 0921620070370040241
|
|
Journal of Bacteriology and Virology
2007 Volume.37 No. 4 p.241 ~ p.248
|
|
|
The Expression of TLR3 and Cytokines Induced by Poly I:C in Human Retinal Pigment Epithelial Cells
|
|
Choi Sun-Ju
Kim Jong-Wook
Park Su-Jung
Park Hyun-Sook
Lee Kyoung-Ho
Kim Soo-Kie
Park Joo-Young
|
|
|
Abstract
|
|
|
|
In this study, we examined the expression of Toll-like receptor3 (TLR3) by human retinal pigment epithelial cells (RPE) and determined whether exposure to the TLR3 agonist polyinosinic-polycytidylic acid (poly I:C) would induced the expression of cytokines in these cells. RT-PCR revealed that TLR3 was constitutively expressed in human RPE, and its expression was increased by treatment with poly I:C. After treatment with poly I:C, we determined the expression levels of pro-inflammatory cytokines in human RPE using RT-PCR and ELISA. We demonstrated that poly I:C treatment increased the production of TNF-alpha, IL-6, and IL-8 in human RPE. Upon exposure to poly I:C, human RPE initiated antiviral response resulting in the induction of IFN-beta mRNA expression and 2’,5’-oligoadenylate synthetase mRNA expression. These results suggest that human RPE may participate in ocular defense mechanism against viral infection through TLR3.
|
|
|
KEYWORD
|
|
|
Toll-Like receptor3, Poly I:C, Viral infection, Retinal pigment epithelium, Interferon
|
|
|
FullTexts / Linksout information
|
|
|
|
|
Listed journal information
|
|
 
|